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Fig. 9 | BMC Research Notes

Fig. 9

From: Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco

Fig. 9

Agarose gel electrophoresis of one-step RT-PCR amplified products of the IBV S1 gene. Commercial IBV vaccine (IBV H120, MA5, and 4/91) and field strains (“Moroccan 30” and “Moroccan 38”) were separated by electrophoresis on a 1.5 % agarose gel stained with ethidium bromide. Lane 1 100 bp DNA ladder; lane 2 Moroccan 30 strain; lane 3 negative control; lane 4 Moroccan 38 strain; lanes 5, 6 negative controls; lane 7 Ma5 commercial vaccine; lane 8 H120 commercial vaccine; lane 9 4/91 commercial vaccine. Product size, 700 bp. Bp base pairs, IBV infectious bronchitis virus, RT-PCR reverse transcriptase-polymerase chain reaction

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