cDNA concentration (log10 copies/µl) | Detection method | ||||||
---|---|---|---|---|---|---|---|
SYBR green I real-time RT-PCR | Conventional RT-PCR | ||||||
Ct value | Sensitivity | Specificity | P/N | P/N | |||
% | CI | % | CI | ||||
101 | 36.31 | 0 | 0–69.5 | 100 | 19.3–100 | − | − |
102 | 33.19 | 33.33 | 5.5–88.4 | 100 | 19.3–100 | + | − |
103 | 26.59 | 66.67 | 11.6–94.5 | 100 | 19.3–100 | + | + |
104 | 24.79 | 100 | 30.5–100 | 100 | 19.3–100 | + | + |
105 | 21.90 | 100 | 30.5–100 | 50 | 8.2–91.8 | + | + |
106 | 17.29 | 100 | 30.5–100 | 0 | 0–80.7 | + | + |