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Table 1 Sensitivity and specificity of Sybr green I real-time RT-PCR in detection of cDNA from the IBV H120 vaccine strain compared to conventional RT-PCR agarose gel electrophoresis

From: Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco

cDNA concentration (log10 copies/µl)

Detection method

SYBR green I real-time RT-PCR

Conventional RT-PCR

Ct value

Sensitivity

Specificity

P/N

P/N

%

CI

%

CI

101

36.31

0

0–69.5

100

19.3–100

−

−

102

33.19

33.33

5.5–88.4

100

19.3–100

+

−

103

26.59

66.67

11.6–94.5

100

19.3–100

+

+

104

24.79

100

30.5–100

100

19.3–100

+

+

105

21.90

100

30.5–100

50

8.2–91.8

+

+

106

17.29

100

30.5–100

0

0–80.7

+

+

  1. CI confidence interval, Ct average cycle threshold, RT-PCR reverse transcriptase-polymerase chain reaction, + positive (P), − negative (N)