Skip to main content

Table 2 Validation of the modified antimicrobial susceptibility testing assay employing the DNA binding fluoroprobe Syto9

From: Assessment of free fatty acids and cholesteryl esters delivered in liposomes as novel class of antibiotic

Strainsa

Antibiotic

MIC (μg/mL)b

Expectedc

Measured

 

Standard assay

Modified assay

S. aureus ATCC 29213

Oxacillin

0.12–0.5

0.0625

0.5

Cefotaxime

1–4

1–2

2

Tetracycline

0.12–1

0.125–0.25

0.5

Ciprofloxacin

0.12–0.5

0.25–0.5

0.5

E. faecalis ATCC 29212

Oxacillin

8–32

0.5–2

8–16

Tetracycline

8–32

16

16

Ciprofloxacin

0.25–1

0.5–1

2

E. coli ATCC 25922

Cefotaxime

0.03–0.12

0.125

0.125

Tetracycline

0.5–2

1

2–4

Ciprofloxacin

0.004–0.015

0.0156

0.0156

P. aeruginosa ATCC 27853

Cefotaxime

8–32

8

16

Tetracycline

8–32

16

16–32

Ciprofloxacin

0.25–1

0.25

0.5–1

  1. aDesignated ATCC quality control strains for antimicrobial susceptibility testing
  2. bShown are the values derived from two independent experiments each conducted in triplicate
  3. cAccording to Clinical and Laboratory Standards Institute M100-S22 for broth microdilution assay