Fig. 3
Quetiapine (QTP) reproducibly rescues viability and OL differentiation in the presence of cytokine-mediated toxicity. Expanded OPCs were plated in 96 well plates in DMEM differentiation media for 24 h and either treated for 1 h with 1.1 µM quetiapine or 0.1 % DMSO vehicle, followed by 48 h of 1 ng/ml TNFα + 10 U/ml INFγ insult. A Comparison of MBP expression in TNFα+INFγ or TNFα+INFγ+QTP treated differentiating OLs. OLs were immunostained for MBP (green) and nuclei stained with DAPI (blue). B, C The dose–response relationships and curves were determined and scaled to DMSO (0 % and no insult (100 %). The EC50 values for QTP were calculated to be 157 nM (n = 4, mean ± SEM) for cellular viability and 125 nM (n = 4, mean ± SEM) for OL differentiation. D, E Viability and OL differentiation raw values of negative control DMSO and positive control QTP demonstrate the reproducibility of QTP protection from cytokine toxicity (n = 13, mean ± SEM). Coefficient of variation (CV) values for cell viability were 6.14 and 7.92 % and for OL differentiation were 19.16 and 17.21 % for DMSO and QTP, respectively. CV values <20 % were considered in the acceptable range