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Fig. 1 | BMC Research Notes

Fig. 1

From: A high throughput drug screening assay to identify compounds that promote oligodendrocyte differentiation using acutely dissociated and purified oligodendrocyte precursor cells

Fig. 1

Flow scheme of the acute OL differentiation assay. a Purified oligodendrocyte precursor cells (OPCs) were cultured from P7 rat cortex and plated into PDL/laminin pre-coated 96-well plates. b One hour later test compounds were added. c OPC cultures with compound were maintained in culture for an additional 4 days. d On the 5th day with compound, cells were fixed and immunostained with anti-MBP antibodies and DAPI. e Images were acquired with automated fluorescence microscopy and scored phenotypically for OL differentiation (see “Methods” section)

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