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Table 2 Comparison of RNA QC using five RNA isolation protocols

From: Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

RNA isolation protocol

RIN value

OD 260/280 ratio

OD 260/230 ratio

Number of Tempus tubes

(a) Adult blood

 MagMAX semi-automated

7.85 ± 0.13

2.09 ± 0.01

1.97 ± 0.05

12

 MagMAX manual

7.20 ± 0.11

2.12 ± 0.01

1.19 ± 0.12*

12

 Norgen

7.36 ± 0.12

2.12 ± 0.02

1.76 ± 0.07*

12

 Tempus spin

8.97 ± 0.05

2.09 ± 0.01

2.08 ± 0.01

12

 Tempus 6-port

8.38 ± 0.08

2.20 ± 0.06

2.02 ± 0.05

11

 Average

7.93 ± 0.10

2.12 ± 0.01

1.80 ± 0.05

59

(b) Cord blood

 MagMAX semi-automated

7.47 ± 0.22

2.09 ± 0.03

2.01 ± 0.09

3

 MagMAX manual

7.73 ± 0.47

2.12 ± 0.01

2.14 ± 0.02

3

 Norgen

8.23 ± 0.33

2.11 ± 0.01

2.07 ± 0.04

3

 Tempus spin

7.13 ± 0.13

2.10 ± 0.01

2.11 ± 0.01

3

 Tempus 6-port

8.37 ± 0.38

2.10 ± 0.01

2.10 ± 0.02

6

 Average

7.88 ± 0.19

2.10 ± 0.02

2.09 ± 0.05

18

  1. * RNA extracted with MagMAX manual and Norgen protocols have significantly lower OD 260/230 ratio compared to RNA from the other protocols, p < 0.05
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BMC Research Notes

ISSN: 1756-0500

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