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Table 4 Raw Cq-value and CVs (coefficients of variation) for cord blood samples

From: Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

RNA isolation protocol CDKN1A FOS IL1B IL8 MYC TP53
MagMax semiautomated
 Average 32.5 28.0 29.8 32.2 29.9 29.5
 SD 0.5 0.9 0.6 1.2 0.3 0.2
 % CV 1.4 3.2 2.0 3.8 1.1 0.7
MagMax manual
 Average 32.0 28.2 29.5 32.6 29.4 29.1
 SD 0.2 0.3 0.3 0.4 0.2 0.2
 % CV 0.6 1.1 1.1 1.3 0.7 0.6
Norgen
 Average 32.7 28.2 29.7 31.5 30.0 29.6
 SD 0.6 1.4 1.0 2.4 0.3 0.5
 % CV 1.8 5.1 3.5 7.5 1.1 1.6
Tempus spin
 Average 31.8 28.0 28.8 31.2 29.3 28.7
 SD 0.2 0.3 0.2 0.4 0.1 0.2
 % CV 0.7 1.2 0.7 1.4 0.4 0.7
Tempus 6-port
 Average 33.0 29.0 30.1 32.8 31.0 29.9
 SD 0.6 0.5 0.3 0.4 0.8 0.4
 % CV 1.8 1.8 0.9 1.3 2.5 1.2
Overall average
 Average 32.5 28.4 29.7 32.2 30.1 29.5
 SD 0.6 0.9 0.7 1.3 0.8 0.5
 % CV 2.0 3.0 2.3 3.9 2.8 1.8
  1. CVs are shown in italics; the overall averaged CVs range between 1.8 and 3.9 %. The average CV value of the IL8 gene isolated with the Norgen protocol had the highest CV value (CV = 7.5 %) and shown in bolditalics