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Table 4 Raw Cq-value and CVs (coefficients of variation) for cord blood samples

From: Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank

RNA isolation protocol

CDKN1A

FOS

IL1B

IL8

MYC

TP53

MagMax semiautomated

 Average

32.5

28.0

29.8

32.2

29.9

29.5

 SD

0.5

0.9

0.6

1.2

0.3

0.2

 % CV

1.4

3.2

2.0

3.8

1.1

0.7

MagMax manual

 Average

32.0

28.2

29.5

32.6

29.4

29.1

 SD

0.2

0.3

0.3

0.4

0.2

0.2

 % CV

0.6

1.1

1.1

1.3

0.7

0.6

Norgen

 Average

32.7

28.2

29.7

31.5

30.0

29.6

 SD

0.6

1.4

1.0

2.4

0.3

0.5

 % CV

1.8

5.1

3.5

7.5

1.1

1.6

Tempus spin

 Average

31.8

28.0

28.8

31.2

29.3

28.7

 SD

0.2

0.3

0.2

0.4

0.1

0.2

 % CV

0.7

1.2

0.7

1.4

0.4

0.7

Tempus 6-port

 Average

33.0

29.0

30.1

32.8

31.0

29.9

 SD

0.6

0.5

0.3

0.4

0.8

0.4

 % CV

1.8

1.8

0.9

1.3

2.5

1.2

Overall average

 Average

32.5

28.4

29.7

32.2

30.1

29.5

 SD

0.6

0.9

0.7

1.3

0.8

0.5

 % CV

2.0

3.0

2.3

3.9

2.8

1.8

  1. CVs are shown in italics; the overall averaged CVs range between 1.8 and 3.9 %. The average CV value of the IL8 gene isolated with the Norgen protocol had the highest CV value (CV = 7.5 %) and shown in bolditalics