Skip to main content

Table 1 Liver Cyp2a5 (COH), Cyp1a1/2 (EROD) and Cyp2b9/10 (BROD) activities in different mouse strains. Female mice were treated ip with PBS (CON), pyrazole (PYR 100 mg/kg bw/days × 3 days), or phenobarbital (PB, 80 mg/kg bw/days × 3 days) and euthanized 24-h after the last dose

From: Interstrain differences in the expression and activity of Cyp2a5 in the mouse liver

Treatment

Monooxygenase activity (pmoles/mg protein/min)

COH

EROD

BROD

CON

PYR

PB

CON

PYR

PB

CON

PYR

PB

Mouse strain

 BALB/cAn

35.2 ± 2.9

69.7 ± 4.4*

75.3 ± 6.0*

71.2 ± 6.0

103.1 ± 9.0

532.7 ± 77.5*

56.6 ± 5.7

53.3 ± 4.0

192.8 ± 23.9*

 CBA

32.9 ± 5.0

119.7 ± 5.2*

91.9 ± 5.4*

74.9 ± 4.9

61.5 ± 5.5

293.0 ± 33.7*

22.0 ± 2.3

19.8 ± 3.5

212.5 ± 22.4*

 C57BL/6

44.5 ± 6.0

112.1 ± 8.4*

62.2 ± 5.5

99.2 ± 7.2

98.7 ± 8.6

444.3 ± 28.6*

47.3 ± 5.8

33.4 ± 4.8

158.9 ± 8.7*

 C57BL/10

24.4 ± 3.8

88.1 ± 4.1*

63.6 ± 5.9*

132.2 ± 9.4

135.5 ± 16.9

598.7 ± 59.5*

42.6 ± 3.5

72.9 ± 29.9

181.3 ± 5.5*

 DBA-1

142.5 ± 28.3

344.8 ± 50.6*

261.8 ± 24.6

50.2 ± 3.3

50.0 ± 4.0

265.7 ± 13.1*

26.8 ± 1.4

43.4 ± 12.4

271.0 ± 2.3*

 DBA-2

228.4 ± 24.8

507.7 ± 12.5*

493.5 ± 11.7*

74.9 ± 7.9

64.3 ± 6.3

338.1 ± 14.8*

39.8 ± 2.8

41.0 ± 3.8

266.9 ± 28.5*

 B6D2F1

113.3 ± 11.6

437.8 ± 24.3*

308.4 ± 20.6*

68.8 ± 3.4

66.6 ± 3.6

251.1 ± 14.1*

35.2 ± 4.1

45.0 ± 5.3

306.5 ± 8.8*

 SW

24.1 ± 5.3

78.5 ± 8.8*

42.5 ± 3.1

72.8 ± 7.6

74.8 ± 9.1

348.5 ± 29.7*

41.2 ± 7.8

38.8 ± 10.2

143.6 ± 4.4*

  1. Values are shown as mean ± SEM. Data were evaluated by ANOVA and Dunnett’s post hoc test. Means that are different (P < 0.05) from respective controls are indicated by an asterisk (*). N = 6 mice of each strain per group (CON, PYR or PB)
  2. COH (coumarin 7-hydroxylase) pmoles umbelliferone/mg protein/min, EROD (ethoxyresorufin-), BROD (benzyloxy-resorufin-O-dealkylase) pmoles resorufin/mg protein/min