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Table 2 RNA isolation from PCLS from different species using an optimized protocol

From: RNA isolation from precision-cut lung slices (PCLS) from different species

Species

Incu-bation period

RNA yield (µg)

Absorbance 260/280

RIN value

Mean

Min.

Mean

Min.

p value

Mean

Min.

p value

Human (n = 12)

w/o

1.12 ± 0.44

0.62

1.91 ± 0.07

1.79

 

8.9 ± 0.7

7.8

 

Human, donor 1 (n = 12)

3 days

2.34 ± 1.03

1.34

1.90 ± 0.04

1.81

0.9107

8.7 ± 0.3

8.1

0.2691

Human, donor 2 (n = 12)

3 days

1.52 ± 0.15

1.29

1.91 ± 0.09

1.75

0.4783

9.0 ± 0.4

8.6

0.7983

Human, donor 3 (n = 12)

3 days

0.98 ± 0.28

0.56

1.88 ± 0.11

1.77

0.9701

9.0 ± 0.5

8.4

0.8112

Rhesus (n = 5)

w/o

2.02 ± 0.37

1.58

1.91 ± 0.06

1.87

 

8.2 ± 0.5

7.4

 

Marmoset (n = 5)

w/o

1.37 ± 0.16

1.16

1.87 ± 0.07

1.79

 

8.2 ± 0.3

7.8

 

Rat (n = 5)

w/o

3.80 ± 0.78

2.48

1.95 ± 0.04

1.91

 

8.3 ± 1.0

6.8

 

Mouse (n = 5)

w/o

5.67 ± 0.61

4.65

1.95 ± 0.01

1.93

 

8.2 ± 0.5

7.3

 
  1. The average is indicated ± STABW. Student’s t-test was performed to compare absorbance 260/280 and RIN values with and without an incubation period, p-values are indicated
  2. n indicates the number of analysed samples, w/o without