Table 1 Primers used in the PCR–RFLP analyses
Pfcrt | |
 1st amplification | |
  pfcrt76F1st | |
  GCGCGCGCATGGCTCACGTTTAGGTGGAG | |
  pfcrt76R1st | |
  GGGCCCGGCGGATGTTACAAAACTATAGTTACC | |
 2nd amplification for PCR–RFLP | |
  pfcrt76F2nd | |
  TGTGCTCATGTGTTTAAACTT | |
  pfcrt76R2nd | |
  CAAAACTATAGTTACCAATTTTG | |
 2nd amplification for NGS amplicon sequencing | |
  pfcrt76F2ndNGStag | |
  ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNTGTGCTCATGTGTTTAAACTT | |
  pfcrt76R2ndNGStag | |
  GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNCAAAACTATAGTTACCAATTTTG | |
pfmdr1 | |
 1st amplification | |
  pfmdr86F | |
  GCGCGCGTTGAACAAAAAGAGTACCGCTG | |
  pfmdr86R | |
  GGGCCCTCGTACCAATTCCTGAACTCAC | |
 2nd amplification for PCR–RFLP | |
  pfmdr86F | |
  TTTACCGTTTAAATGTTTACCTGC | |
  pfmdr86R | |
  CCATCTTGATAAAAAACACTTCTT | |
 2nd amplification for NGS amplicon sequencing | |
  pfmdr86F2ndNGStag | |
  ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNACCGTTTAAATGTTTACCTGC | |
  pfmdr86R2ndNGStag | |
  GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNCATCTTGATAAAAAACACTTCTT |