Fig. 2

The expression of rhGM-CSF in P. pastoris analyzed by SDS-PAGE (a) and immunoblot probed with an anti-hGM-CSF antibody (b). M, molecular mass markers; 1, P. pastoris X33 culture supernatant 72 h post-induction with methanol; 2, P. pastoris X33::hgm-csfculture supernatant 72 h post-induction; 50 µL crude supernatant was precipitated and loaded per lane