Fig. 3

The expression of rhGM-CSF in S. cerevisiae analyzed by SDS-PAGE (a) and immunoblot probed with an anti-hGM-CSF antibody (b). M molecular markers; 1 supernatant from S. cerevisiae BY4742 72 h post-induction with galactose; 2, 3 and 4, supernatant from S. cerevisiae BY4742/pYES2-hgm-csf induced with galactose after 24, 48 and 72 h, respectively 50 µL crude supernatant was precipitated and loaded per lane