Fig. 4From: Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factorThe secreted expression of rhGM-CSF analyzed by SDS-PAGE; hours post induction (h.p.i) are indicated for P. pastoris X33::hgm-csf induced with methanol (a) or S. cerevisiae BY4742/pYES2-hgm-csf induced with galactose (b). M molecular mass markers; 50 µL crude supernatant was precipitated and loaded per laneBack to article page