Fig. 6From: Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factorPurification of rhGM-CSF from culture supernatants of P. pastoris X33::hgm-csf induced with methanol analyzed by SDS-PAGE. a Hydrophobic interaction chromatography. M molecular mass markers, S supernatant after 72 h, F column flow-through, E eluted fraction. b Ion exchange chromatography. M molecular mass markers, S supernatant after 72 h, F column flow-through, E1–6 fractions eluted with NaCl (50, 100, 200, 400, 600 and 1000 mM, respectively)Back to article page