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Fig. 6 | BMC Research Notes

Fig. 6

From: Pichia pastoris versus Saccharomyces cerevisiae: a case study on the recombinant production of human granulocyte-macrophage colony-stimulating factor

Fig. 6

Purification of rhGM-CSF from culture supernatants of P. pastoris X33::hgm-csf induced with methanol analyzed by SDS-PAGE. a Hydrophobic interaction chromatography. M molecular mass markers, S supernatant after 72 h, F column flow-through, E eluted fraction. b Ion exchange chromatography. M molecular mass markers, S supernatant after 72 h, F column flow-through, E16 fractions eluted with NaCl (50, 100, 200, 400, 600 and 1000 mM, respectively)

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