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Fig. 4 | BMC Research Notes

Fig. 4

From: Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site

Fig. 4

Scheme for recovery of BAC deletions. The transposon plasmid is introduced into the cell harboring the BAC clone of interest. Random transposition of the mini Tn10 cassette into the BAC clone is initiated upon the addition of IPTG. P1vir s infection results in the deletion of the DNA between two mutant loxP sites in the same orientation and formation of a P1-BAC cointegrate. P1 packaging initiates at the pac site until the capsid is full. Infection of the Cre+ strain NS3516 results in cyclization of the BAC clone

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