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Table 1 Real-time PCR amplification scores versus visual observations

From: The detection of great crested newts year round via environmental DNA analysis

 

eDNA score 1

eDNA score 2

eDNA score 3

Visual observation

Pond 1 March

7/12

3/12

6/12

Present

Pond 2 March

11/12

11/12

12/12

Present

Pond 1 April

12/12

12/12

11/12

Present

Pond 2 April

12/12

12/12

11/12

Present

Pond 1 May

6/12

9/12

3/12

Present

Pond 2 May

11/12

9/12

9/12

Present

Pond 1 June

5/12

4/12

4/12

Present

Pond 2 June

12/12

11/12

12/12

Present

Pond 1 July

6/12

11/12

9/12

Present

Pond 2 July

12/12

12/12

12/12

Present

Pond 1 August

7/12

7/12

8/12

Present

Pond 2 August

12/12

12/12

11/12

Present

Pond 1 September

1/12

0/12

0/12

Absent

Pond 2 September

3/12

2/12

0/12

Absent

Pond 1 October

0/12

0/12

0/12

Absent

Pond 2 October

0/12

2/12

0/12

Absent

Pond 1 November

0/12

1/12

0/12

Absent

Pond 2 November

1/12

4/12

1/12

Absent

Pond 1 December

1/12

0/12

1/12

Absent

Pond 2 December

0/12

0/12

0/12

Absent

Pond 1 January

6/12

0/12

3/12

Absent

Pond 2 January

2/12

3/12

4/12

Absent

Pond 1 February

6/12

4/12

5/12

Present

Pond 2 February

2/12

0/12

0/12

Present

  1. Real-time PCR amplification scores (numbers of individual PCRs positive out of a maximum of 12 replicates) for eDNA extracted from water samples taken monthly from two ponds over a year. eDNA was measured using PCR × 12 replicates for each of three separate PCR amplifications of a single eDNA extraction. Visual observations were made prior to collection of the water samples every month and great crested newts recorded as present or absent