Fig. 5
NGS and Sanger sequencing results at chromosome 4, location 72611690. A tri-allelic variant was identified at this locus. The variant was reported by the Ion S5â„¢ XL variant caller file but not the Ion PGMâ„¢ variant caller file despite appearing in both in Partek Genomics Suiteâ„¢ (on left). Sanger sequencing on the right for sample LT1308 as well as two other samples (LT744, LT120) shows that sample LT1308 is a T/C heterozygote while sample LT744 is G/G and sample LT120 is C/C