Fig. 6
NGS and Sanger sequencing results at chromosome 10, location 16911556. At this locus, a heterozygote G/T variant was called in sample LT1308 by the Ion S5â„¢ XL but not the Ion PGMâ„¢. Visual inspection of the Sanger sequencing results (shown here on the reverse strand) determined that the variant had been called incorrectly. There is a peak shown for A which matches the reference sequence. It is likely that the homo-polymer region was the reason the Ion S5â„¢ XL had difficulty reporting this SNP