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Table 3 Sequencing runs completed using both the Ion PGM™ and Ion S5™ XL

From: Design and implementation of a custom next generation sequencing panel for selected vitamin D associated genes

Sequencing Runs per chip
Sequencer Chip type Dilution (pM) Preparation Max. output Samples Usable reads (%) Max. reads Polyclonal (%)
Ion PGM™ Ion 316™ 26 Ion Chef™ 429 Mb 8–12 51–57 2,755,634 21–37
Ion PGM™ Ion 318™ 26/50 Ion Chef™/Manual 668 Mb 48–96 48–50 4,880,500 11–40
Ion S5™ XL Ion 530™ 50 Ion Chef™ 3.22 Gb 85 50–61 80,308,654 26–30
  1. Libraries were prepared using 20 ng genomic DNA. Dilution factors chosen for the sequencing runs were either 26 or 50 pM depending on the number of samples to be sequenced and the level of coverage desired
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