Fig. 2

Detection of the α1 chain of collagen VI. Immunostaining was conducted on cross- (A, E, I, M, Q) and transverse (B, F, J, N and R) sections of normal human skeletal muscle. Antibodies are listed on left. Blots containing reduced and non-reduced sets of lysates from cells expressing α1, α2 and α3 chains individually (C, G, K, O and S) were probed with each α1 antibody. Blots containing lysates from cells expressing multimeric collagen VI containing all three chains were resolved under non-reducing conditions and probed (D, H, L, P and T). Migration position of molecular weight markers (in kDa) is shown between each pair of immunoblots