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Table 2 Summary of immunohistochemistry and immunoblot results

From: Authentication of collagen VI antibodies

Antibody

Cat number

Signal by IHC

α1 lysate

α2 lysate

α3 lysate

α1 α2 α3 lysate

Figures

COL6A1 H200

sc-20649

++

++ (R)

−

−

+

1

COL6A1

ab199720

+++

+++ (R)/+++ (NR)

−

−

+

1

COL6A1

ab182744

++

++ (R)

−

−

+

1

COL6A1

17023-1-AP

+++

+++ (R)/+++ (NR)

−

−

+

1

COL6A1 B4

sc-377143

−

+++ (R)

−

−

+

1

COL6A2

14853-1-AP

++

−

++ (R)/+ (NR)

−

nd

2

COL6A2 EPR7889

ab180855

+++

−

+++ (R)

−

−

2

COL6A2

ab172606

++

−

+ (R)

−

nd

2

COL6A2 D20

sc-83607

+++

−

+++ (R)

−

++

2

COL6A2 H300

sc-292186

++

−

+ (R)

−

+

2

COL6A2 K15

sc-377143

+++

−

+ (R)

−

+

2

COL6A2 B7

sc-374566

−

−

+++ (R)/+ (NR)

−

++

2

COL6A3 G18

sc-131139

++

−

−

+ (NR)

+

3

COL6A3 3C4

MAB1944

+++

−

−

+ (R)/++ (NR)

−

3

COL6A3 3C4

sc-47712

 

−

−

+

nd

3

COL6A3 H300

sc-367543

+++

−

−

−

−

3

COL6A3 H3-2

sc-81766

−

−

−

−

−

3

COL6A3 64C11

ab49273

−

−

−

−

−

3

COL6A3 N12

sc-131140

−

−

−

−

nd

3

Collagen VI VI-26

MAB3303

+++

−

−

−

+

4

Collagen VI

70R-CR009X

+++

−

−

++ (NR)

+++

4

Collagen VI 172C2

sc-47764

−

−

−

++ (NR)

−

4

Collagen VI 5C6

 

−

−

−

−

+

4

Collagen VI

AB7821

−

−

Non-sp.

Non-sp.

++

4

  1. Each antibody is scored for signal intensity on immunohistochemical signal and, for immunoblots, band intensity ranging from ‘−’ representing no detectable signal, to ‘+++’ representing strong signal. The description of immunoblots signal strength scores reduced (R) and non-reduced (NR) samples separately. Some blots on triple transfected lysates were not conducted and are designated as not determined (nd)