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Fig. 2 | BMC Research Notes

Fig. 2

From: Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris

Fig. 2

Expression of the S-HDAg-His6-tag fusion protein. An SDS-PAGE gel image showing the expression of the S-HDAg-His6-tag fusion protein after a 24-h, b 48-h and c 72-h induction. Lanes 1–3 indicate three colonies, of which lanes 1 and 3 show recombinant protein expressed after 48-h and 72-h induction; M are Protomarker Protein Markers (National Diagnostics). Protein was probed with a 6× His monoclonal antibody (Serotec)

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