Skip to main content

Table 1 Performance of Vikia® Rota-Adeno and Premier™ Rotaclone ® compared to RT-PCR on frozen samples

From: Diagnostic accuracy of VIKIA® Rota-Adeno and Premier™ Rotaclone® tests for the detection of rotavirus in Niger

 

RT-PCR

Test performance

Positive

Negative

Total % sensitivity (95% CI) % specificity (95% CI)

Vikia Rota-Adeno

80.7 (72.4–87.3) 95.5 (90.4–98.3)

 Positive

96

6

102

 Negative

23

126

149

Premier Rotaclone

80.7 (72.4–87.3) 100 (97.2–100)

 Visual reading

  Positive

96

0

96

  Negative

23

131

154

  Indeterminate

0

1

1

 Optical density

  Positive

96

0

96

  Negative

23

132

155

Total

119

132

251

  1. In order to assess possible sample selection bias and freezing/thawing impact on sample rotavirus status, a comparison of the initial VIKIA® Rota-Adeno test in the field for the purposes of surveillance with RT-PCR results on all specimens tested and those selected for the evaluation are shown in Table 2. There was no significant difference between the sensitivity and specificity of the RDT when calculated using all specimens or only those selected for the evaluation, suggesting absence of sample selection bias