Fig. 2
a Graphical summary of changes in Complex 1 subunits of the liver in donor after brainstem death v donor after cardiac death. Changes in levels of Complex 1 subunits are expressed as a ratio to β-actin comparing expression level of given subunit in donor after brainstem death (DBD, n = 4) compared to donor after cardiac death (DCD, n = 6) liver section. Complex 1 subunits compared were NDUFB6 (distal part of membrane arm), NDUFV2 (distal part of matrix arm), NDUFA10 (middle part of membrane arm) and MTND1 (proximal part of membrane arm). Y-axes represent given Complex 1 subunit signal relative to β-actin signal. Error bars indicate standard deviation. No significant difference was demonstrated. b Graphical summary of changes in Complex 1 subunits steatotic v non steatotic liver. Changes in levels of Complex 1 subunits are expressed as a ratio to β-actin comparing expression level of given subunit in the non steatotic (n = 4) compared to steatotic (n = 6) donor liver section. Complex 1 subunits compared were NDUFB6 (distal part of membrane arm), NDUFV2 (distal part of matrix arm), NDUFA10 (middle part of membrane arm) and MTND1 (proximal part of membrane arm). Y-axes represent given Complex 1 subunit signal relative to β-actin signal. Error bars indicate standard deviation. No significant difference was demonstrated. c Graphical summary of changes in Complex 1 subunits O2 v non O2 exposed section of liver. Changes in levels of Complex 1 subunits are expressed as a ratio to β-actin comparing expression level of given subunit in the section of liver exposed to oxygen (O2) via anterograde persufflation (n = 5) compared to the control section of liver subjected to cold static storage (n = 5). Complex 1 subunits compared were NDUFB6 (distal part of membrane arm), NDUFV2 (distal part of matrix arm), NDUFA10 (middle part of membrane arm) and MTND1 (proximal part of membrane arm). Y-axes represent given Complex 1 subunit signal relative to β-actin signal. Error bars indicate standard deviation. No significant difference was demonstrated