Skip to main content
Fig. 1 | BMC Research Notes

Fig. 1

From: The analysis of novel microRNA mimic sequences in cancer cells reveals lack of specificity in stem-loop RT-qPCR-based microRNA detection

Fig. 1

Determining specificity of assays specific for consensus mimics with total RNA as template. a Experimental design, in which each RT primer is combined with each TaqMan assay; only the specific combination of RT primer and TaqMan assay for miR-16 (all coloured red) should produce a signal in RNA from untransfected cells. b RT-qPCR data produced with each RT primer/TaqMan assay combination using RNA isolated from untransfected MSTO and VMC23 cells. Data are raw Cq values and the mean ± SD of three replicate experiments

Back to article page