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Fig. 1 | BMC Research Notes

Fig. 1

From: Extracellular cathepsin L stimulates axonal growth in neurons

Fig. 1

Effect of extracellular cathepsin L on densities of axons and dendrites. Cortical neurons (a, b, e) and spinal cord neurons (c, d, f) were cultured for 3 days and then treated with vehicle solution (Cont) or cathepsin L (at a dose of 1, 10, 50, or 100 ng/ml). The cells were fixed and double-immunostained for against phosphorylated neurofilament-H (pNF-H) and MAP2 antibodies, 4 days after the treatment. The density of pNF-H-positive axons (a, c) and microtubule-associated protein 2 (MAP2)-positive dendrites (b, d) were quantified (**p < 0.01, one-way ANOVA with post hoc Dunnett’s test, n = 12–20 photos). The numbers of cortical neurons (e) and spinal cord neurons (f), which were MAP2-positive, were quantified (one-way ANOVA with post hoc Dunnett’s test, n = 12–20 photos)

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