Fig. 2From: Optimization of PCR conditions for amplifying an AT-rich amino acid transporter promoter sequence with high number of tandem repeats from Arabidopsis thaliana Effects of MgCl2 concentration on PCR amplification at an extension temperature of 65 °C. Lane M: 10 kb DNA ladder; lane 1: 1.5 mM MgCl2; lane 2: 2 mM MgCl2; lane 3: 2.5 mM MgCl2; lane 5: 3 mM MgCl2; lane 6: 3.5 mM MgCl2; lane 7: no-template negative controlBack to article page