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Fig. 3 | BMC Research Notes

Fig. 3

From: Human regulatory proteins associate with non-coding RNAs from the EBV IR1 region

Fig. 3

RIP binding of ncRNA from EBV W-repeat region. a Fold enrichment for ebv-sisRNA-1 or ebv-sisRNA-2 after RIP using antibodies against the indicated proteins from BJAB-B1 or Raji cells. b Fold enrichment for ebv-sisRNA-1 to exon W2 (a3 from Fig. 2a) after RIP using antibodies against the indicated proteins from BJAB-B1 or Raji cells. For all RIPs, data represent the mean of mostly two independent experiments per cell line (where only one experiment was done is shown in Additional file 3) and data are normalized to control IgG. For both A and B, data are cut off at a fold enrichment value of 25. c RT-PCR (a4 from Fig. 2a) from either lentiviral transduced BJAB using empty vector or a W1 through W2 overexpression (OE) construct or from two independent RNA isolations from BJAB-B1 cells. The amplified forms of the unspliced (u) or spliced (s) products are indicated. d RT-PCR (a5 from Fig. 2a) from BJAB, BJAB-B1, or Raji total RNA across the exon/intron boundaries (a5 from Fig. 2a) for retention of sisRNA-1. The amplified forms of the unspliced (u) or spliced (s) products are indicated

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