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Long-term effects on the progress of neuropathy after diabetic Charcot foot: an 8.5-year prospective case–control study
BMC Research Notes volume 11, Article number: 140 (2018)
Charcot foot is a severe complication to diabetes mellitus, associated with diabetic neuropathy. Any long-term effects of a Charcot foot on the progress of neuropathy are still largely unexplored. The objective was to investigate whether a previous Charcot foot had any long-term effects on the progress of neuropathy.
An 8.5-year follow-up case–control study of 49 individuals with diabetes mellitus, 24 of whom also had Charcot foot at baseline visit in 2005–2007. Neuropathy was assessed with a questionnaire, biothesiometry, heart rate variability and venous occlusion plethysmography. Of the 49 baseline participants, 22 were able to participate in the follow-up. Twelve had passed away in the meantime. Heart rate variability was unchanged in both groups; from 9.7 to 7.2 beats/min (p = 0.053) in the Charcot group, and 14.3 to 12.6 beats/min (p = 0.762) in the control group. Somato-sensoric neuropathy showed no difference between baseline and follow-up in the Charcot group (from 39.1 to 38.5 V) (p = 0.946), but a significantly worsened sensitivity in the control group (from 25.1 to 38.9 V) (p = 0.002). In conclusion, we found that any differences in somatic or cardial autonomic neuropathy present at baseline had disappeared at follow-up after 8.5 years.
Charcot foot (CF) is an unusual bone and joint disorder which causes hyperemia, inflammation and degeneration of the weight-bearing structures in the foot. If left untreated it can result in spontaneous fatigue bone fractures, leading to deformity and ulcerations [1, 2]. Although the precise pathological mechanism underlying the Charcot foot is unknown, it is exclusively seen in individuals with established peripheral neuropathy [3,4,5]. Most cases in the Western world occur in patients with diabetes mellitus, with an incidence rate of about 0.3% per year [6, 7].
We have previously shown that local autonomic neuropathy in the feet, assessed through a weakened venoarterial sympathetic axon reflex, is not directly related to the development of an acute Charcot foot, despite the increased blood flow  (compared to diabetic controls with and without somato-sensoric neuropathy). Furthermore, there seemed to be a dissociation between local and central autonomic neuropathy (as measured by heart rate variability through beat-to-beat analysis). This supports the theory that the inflammation is provoked by repeated local microtrauma and bone remodelling, as suggested by others [9,10,11,12].
It is still unknown whether the chronic deformities from a Charcot foot are related to lasting alterations in systemic or local neuropathy. If so, that could account for some of the recurrences of Charcot foot which are so far somewhat unexplained [13, 14].
To investigate this we conducted a follow-up study of a previously well-described group of diabetes patients with or without a Charcot foot [8, 15, 16]. The main goal was to investigate if a former Charcot foot had any long-term effects on somato-sensoric or autonomic neuropathy, including local blood flow.
A total of 49 patients were included at the baseline visit in 2005–2007 [8, 15]. All 49 patients were followed up using medical records, the “Danish Register of Cause of Death” and the “Danish Civil Registration System”. The included patients were 61.7 ± 7.2 years old and were distributed as 40 males and 9 females. Ten patients had type 1 diabetes, and 39 had type 2 diabetes.
At baseline in 2005–2007 all examination were performed by author TMC, and at follow-up by author RBJ. All examinations were performed using the same equipment and the same procedures. Patients were examined in order of their availability over an 11 months period at the Endocrine Research Unit at Bispebjerg Hospital, Denmark.
Physical examinations focused on the lower extremities including deformities, wounds and loss-of-function. The degree of neuropathy was assessed by the modified questionnaire Neuropathy Symptom Score (NSS) (Additional file 1: Appendix S1) [17, 18]. In addition, vibration perception thresholds was measured by biothesiometry (Biothesiometer, Bio-Medical Instruments Co, Newbury, Ohio 44065, USA), with the probe placed on the hallux pulpa [19,20,21].
Cardial Autonomic Neuropathy (CAN) was assessed using the heart rate variability method by utilizing the autonomic cardiac regulation. Measurements were done using PowerLab, Chart 5 v5.5 (ADInstruments Pty Ltd, Castle Hill, NSW, Australia). For electrocardiography measurement three electrodes were placed on the patient’s chest. The patient was instructed to rest for 10 min, after which baseline readings were taken. The patient was then instructed to adjust their respiration rate to 6 steady breaths per minute for 1 min, each inspiration and expiration phase taking 5 s. This was controlled with a stopwatch and under constant instruction and monitoring. The procedure was repeated five times with 5 min of rest between measurements. The measurements selected as results were the ones which were evaluated to display the best compliance to the instructions (based on respiration and heart-rate pattern) .
Blood flow in the feet was measured using venous occlusion plethysmography [23, 24] with a Hokanson EC6 Plethysmograph with a Hokanson E20 Rapid Cuff Inflator (Bellevue, WA, USA). The measurements were done with the patient lying on his/her back after a rest period of 30 min. The cuff was placed around the calf, and a fitting strain gauge was placed around the forefoot. Cuff pressure was 40 mmHg for 7 s, and the inflation was repeated 5 times on each foot.
Data are expressed as means ± 1 Standard Deviation (SD) unless otherwise noted. Normal distribution was controlled with the Shapiro-Wilks tests. T-tests and Mann–Whitney rank sum tests were used for comparisons between two data sets (parametric and non-parametric respectively).
For variance analysis between multiple ranked groups the non-parametric Kruskal–Wallis one way analysis of variance was used. For matched groups (e.g. baseline versus follow-up) paired t-tests and Wilcoxon signed rank tests were used. For compairing categorical data (2 × 2 tables), Chi square test was used. Results were adjusted using Bonferroni correction when applicable (multiple comparisons).
Statistics and generel data handling was done using IBM SPSS Statistics v. 23 by IBM Corporation, SIGMAPLOT v. 188.8.131.52 by Systat Software Inc., Microsoft Excel 2000 v. 9.0.2812 by Microsoft Corporation and Apache OpenOffice 4.0.1 by The Apache Software Foundation.
Of the 49 patients at the baseline visit, 12 had passed away (Table 1 and Additional file 2: Figure S1). Thus, the mortality rate over 8.5 years was about 25%. The causes of death were mainly related to late stage diabetic complications, and were: nephropathy (n = 3), cancer (n = 3), coronary artery disease (n = 2), complicated infections/septicaemia (n = 2), postoperative pulmonary embolism (n = 1) and unknown (n = 1).
We were unable to reach 4 former participants, 3 of whom had moved abroad. Of the remaining 33, 7 didn’t want to participate while 4 had had some form of lower extremity amputation due to diabetic foot ulcers. In total, 22 of the original 49 patients were able to participate in some or all of the follow-up examinations, giving the follow-up visit a participation rate of 45%.
Due to the high loss-to-follow up the participants were pooled into two groups: Diabetes mellitus with or without previous Charcot foot. This distributed the participants in two even groups of 11 each. The group with diabetes and previous Charcot foot is labeled as “DM + CF”, and the group with diabetes but without previous Charcot foot is labeled as “DM–CF”. Antropomorphic data on the participants at the follow-up visit are listed in Table 2.
Data was tested for any possible skewering from a survivor bias due to the high mortality. We pooled the participants with former CF (acute and chronic), and those without to test them in a 2 × 2 table. There was no significant difference in mortality between the two groups (p = 0.863). We divided the baseline population into follow-up (n = 22) and non follow-up participants (n = 27) and tested the two groups against each other for variations regarding age, diabetes age, BMI, HbA1c or biothesiometry without any significant finds (Additional file 3: Table S1).
None of the patients had had a subsequent reactivation of their Charcot foot since the baseline visit. None had active foot ulcers at follow-up either, though some patients in both groups reported having one or more foot ulcers in the follow-up period (n = 6 in the DM + CF group and n = 3 in the DM − CF group).
In the Charcot group, 4 patients had visual foot deformities (assessed without x-ray) after their Charcot foot. All patients with CF were originally treated with off-loading in a removable off-loading cast, worn for an average of 7.7 months (range 16.0 and median 6.0), and all were subsequently provided with some form of special off-loading shoe wear.
The modified NSS at follow-up showed a mean of 3.1 points for all 22 participants, which was not significantly different from the 2.4 points at baseline. Neither the DM + CF nor the DM – CF groups showed a significant difference from baseline to follow-up either (Table 3).
The biothesiometry measurements at baseline showed a significant difference between the average sensitivity in the DM + CF and the DM − CFgroups, with the DM + CF group’s feet having a higher vibration threshold than the DM − CF group’s feet (p = 0.014). The change in sensitivity from baseline to follow-up was significantly increased for the DM − CFgroup compared to the DM + CF group (p = 0.003), to a degree that the two groups had comparable vibration thresholds at follow-up.
CAN was measured in 17 of the 22 participants; 2 wished not to participate while 3 other procedures were unfit for analysis due to a pacemaker (n = 1) or the participants’ inability to cooperate to the measurements (n = 2).
There was no difference in the heart rate variability at baseline between the DM + CF and the DM − CF groups (p = 0.127). The change from baseline to follow-up was unchanged both within the groups and between them (Table 3). In the DM + CF group (n = 11) it changed from 9.7 ± 5.9 to 7.2 ± 4.0 beats/min (p = 0.053), and in the DM − CF group (n = 6) it changed from 14.3 ± 7.5 to 12.6 ± 7.7 beats/min (p = 0.762).
Of the 22 participants, 19 were able to participate to the plethysmography; 1 did not wish to participate while 2 were unable to complete the procedure due to discomfort from the cuff (n = 1), or involuntary muscle cramps which invalidated the measurements (n = 1).
In the DM + CF group (n = 10) there was a significant decrease in blood flow in the previous Charcot foot between baseline and follow-up (p = 0.001), as well as a significant difference in the change from baseline to follow-up between the healthy and affected foot (p = 0.002) (Additional file 4: Figure S2).
There was no difference at follow-up between the blood flow in the DM + CF and DM − CF groups (p = 0.280). However, there was a significant change in the average blood flow in the DM + CF feet compared to the DM − CF from baseline to follow-up (p = 0.009), due to a decrease in blood flow in the DM + CF feet.
In this study we have described a population with diabetes mellitus, half of whom had a Charcot foot 8.5 years ago. We have focused on the possible consequences of this on peripheral sensoric neuropathy, CAN and blood flow in the foot. To our knowledge, this is the first study investigating a population with Charcot foot across those parameters long-term.
We found no differences in somato-sensoric or central autonomic neuropathy had developed during the follow-up period, and that the difference in somatic neuropathy present at baseline had disappeared at follow-up. Baseline data showed a significant difference in both sensitivity and blood flow between the groups, but this had also disappeared at follow-up.
The worsening in sensitivity was only seen in the DM − CF group. The sensitivity in the DM + CF group did not change during the follow-up period, and neither did it differ between the healthy and the affected foot. This suggests that a foot developing an acute Charcot condition is affected equally by neuropathy as the contralateral foot. Overall, there seems to be no lasting effects of an acute Charcot foot on the progress of peripheral somato-sensoric neuropathy or CAN in our population after 8.5 years.
In our population, we see a high mortality in several of the baseline groups (Table 1) which is in line with previously reported mortality rates. As shown by Sohn et al.  people with diabetes have an increased mortality when affected by Charcot foot and/or foot ulcers; both of which are commonly related to neuropathy. Especially diabetic neuropathic foot ulcers are known to be a major cause of mortality. Moulik et al.  report a 5-year mortality of 45%, while Morbach et al. report a cumulative 10-year mortality for people with diabetic foot ulcers of 70% .
Our main findings are that there were no differences between the previous Charcot foot group and control group with regards to somato-sensoric or autonomic neuropathy at follow-up. In fact neuropathy in participants with diabetes but without Charcot foot had significantly worsened from baseline to follow-up, to be comparable to that of those participants with previous acute Charcot foot. In addition, the increased blood flow seen in the acute Charcot foot had, as expected, completely disappeared at follow-up 8.5 years later. Finally, our study confirms the high mortality for individuals with diabetic neuropathy.
The main weakness of the study was the low participation rate in a small population. However, significant dropout and loss-to-follow up is often the case when dealing with patients with a serious disease and its complications. Due to the considerable loss-to-follow up, we chose to include all participants in as many tests as possible, even those who could not participate in all the tests.
cardial autonomic neuropathy
- DM + CF:
diabetes mellitus and Charcot foot
- DM − CF:
diabetes mellitus without Charcot foot
Neuropathy Symptom Score
Lee L, Blume PA, Sumpio B. Charcot joint disease in diabetes mellitus. Ann Vasc Surg. 2003;17(5):571–80.
Rogers LC, Frykberg RG. The charcot foot. Med Clin North Am. 2013;97(5):847–56.
Petrova NL, Edmonds ME. Charcot neuro-osteoarthropathy-current standards. Diabetes Metab Res Rev. 2008;24(Suppl 1):S58–61.
Ward JD. The diabetic leg. Diabetologia. 1982;22(3):141–7.
Rogers LC, Frykberg RG, Armstrong DG, Boulton AJM, Edmonds M, Van GH, et al. The Charcot foot in diabetes. J Am Podiatr Med Assoc. 2011;101(5):437–46.
Hartemann-Heurtier A, Van GH, Grimaldi A. The Charcot foot. Lancet. 2002;360(9347):1776–9.
Fabrin J, Larsen K, Holstein PE. Long-term follow-up in diabetic Charcot feet with spontaneous onset. Diabetes Care. 2000;23(6):796–800.
Christensen TM, Simonsen L, Holstein PE, Svendsen OL, Bülow J. Sympathetic neuropathy in diabetes mellitus patients does not elicit Charcot osteoarthropathy. J Diabetes Complic. 2011;25(5):320–4.
Jeffcoate WJ, Game F, Cavanagh PR. The role of proinflammatory cytokines in the cause of neuropathic osteoarthropathy (acute Charcot foot) in diabetes. Lancet. 2005;366(9502):2058–61.
Jeffcoate WJ. Charcot neuro-osteoarthropathy. Diabetes Metab Res Rev. 2008;24(Suppl 1):S62–5.
Mabilleau G, Edmonds ME. Role of neuropathy on fracture healing in Charcot neuro-osteoarthropathy. J Musculoskelet Neuronal Interact. 2010;10(1):84–91.
Chantelau E, Onvlee GJ. Charcot foot in diabetes: farewell to the neurotrophic theory. Horm Metab Res Horm Stoffwechselforschung Horm Métabolisme. 2006;38(6):361–7.
Christensen TM, Gade-Rasmussen B, Pedersen LW, Hommel E, Holstein PE, Svendsen OL. Duration of off-loading and recurrence rate in Charcot osteo-arthropathy treated with less restrictive regimen with removable walker. J Diabetes Complic. 2012;26(5):430–4.
Osterhoff G, Böni T, Berli M. Recurrence of acute Charcot neuropathic osteoarthropathy after conservative treatment. Foot Ankle Int. 2013;34(3):359–64.
Christensen TM, Bülow J, Simonsen L, Holstein PE, Svendsen OL. Bone mineral density in diabetes mellitus patients with and without a Charcot foot. Clin Physiol Funct Imaging. 2010;30(2):130–4.
Jansen RB, Christensen TM, Bülow J, Rørdam L, Holstein PE, Svendsen OL. Sarcopenia and body composition in diabetic Charcot osteoarthropathy. J Diabetes Complic. 2015;29:937–42.
Dyck PJ, Sherman WR, Hallcher LM, Service FJ, O’Brien PC, Grina LA, et al. Human diabetic endoneurial sorbitol, fructose, and myo-inositol related to sural nerve morphometry. Ann Neurol. 1980;8(6):590–6.
Dyck PJ. Detection, characterization, and staging of polyneuropathy: assessed in diabetics. Muscle Nerve. 1988;11(1):21–32.
Young MJ, Breddy JL, Veves A, Boulton AJ. The prediction of diabetic neuropathic foot ulceration using vibration perception thresholds. A prospective study. Diabetes Care. 1994;17(6):557–60.
Armstrong DG, Lavery LA, Vela SA, Quebedeaux TL, Fleischli JG. Choosing a practical screening instrument to identify patients at risk for diabetic foot ulceration. Arch Intern Med. 1998;158(3):289–92.
Meijer JW, van Sonderen E, Blaauwwiekel EE, Smit AJ, Groothoff JW, Eisma WH, et al. Diabetic neuropathy examination: a hierarchical scoring system to diagnose distal polyneuropathy in diabetes. Diabetes Care. 2000;23(6):750–3.
Ewing DJ, Campbell IW, Murray A, Neilson JM, Clarke BF. Immediate heart-rate response to standing: simple test for autonomic neuropathy in diabetes. Br Med J. 1978;1(6106):145–7.
Whitney RJ. The measurement of volume changes in human limbs. J Physiol. 1953;121(1):1–27.
Zachrisson H, Volkmann R, Bergerheim T, Holm J. Selectivity of superficial vein occlusion at the ankle and calf level: a methodological study in healthy volunteers. Clin Physiol Oxf Engl. 1998;18(1):55–60.
Sohn M-W, Lee TA, Stuck RM, Frykberg RG, Budiman-Mak E. Mortality risk of Charcot arthropathy compared with that of diabetic foot ulcer and diabetes alone. Diabetes Care. 2009;32(5):816–21.
Moulik PK, Mtonga R, Gill GV. Amputation and mortality in new-onset diabetic foot ulcers stratified by etiology. Diabetes Care. 2003;26(2):491–4.
Morbach S, Furchert H, Gröblinghoff U, Hoffmeier H, Kersten K, Klauke G-T, et al. Long-term prognosis of diabetic foot patients and their limbs: amputation and death over the course of a decade. Diabetes Care. 2012;35(10):2021–7.
Author RBJ and TMC contributed to the inclusion of participants, performing examinations and gathering data. Authors RBJ, TMC, JB, LR, PH and OLS contributed equally to the design and setup of experiments, study design and data analyzing. Authors JB and LR also contributed by supplying the equipment used in the study, and technical assistance in its usage. Authors RBJ and OLS contributed equally to the first manuscript draft. Authors TMC, JB, LR and PH all contributed to the revisions, clarifications and manuscript proofreading. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Availability of data and materials
All data and material used will be provided, in anonymized form, upon request to the corresponding author (RBJ) at firstname.lastname@example.org.
Consent for publication
Ethics approval and consent to participate
This study was approved by the Danish comittee for Ethical Science, jr.nr. H-1-2014-055.
All participants gave their informed verbal and written consent to participate before inclusion.
The project was internally funded, none of the authors have any financial interests in the outcome.
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The modified Neuropathy Symptom Score. The questionnaire used to assess patients’ symptoms of neuropathy.
Flowchart of Participants. The flow of participants into the study from baseline to follow-up.
Data comparison between follow-up and lost-to-follow-up. Comparisons between baseline values in the population of former participants lost to follow-up, and participants who joined both baseline and follow-up measurements.
Foot blood-flow. Blood-flow as measured by venous occlusion plethysmography at baseline and at follow-up 8.5 years later for participants with diabetes and an acute or chronic Charcot foot at baseline.
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Jansen, R.B., Møller Christensen, T., Bülow, J. et al. Long-term effects on the progress of neuropathy after diabetic Charcot foot: an 8.5-year prospective case–control study. BMC Res Notes 11, 140 (2018). https://0-doi-org.brum.beds.ac.uk/10.1186/s13104-018-3253-5
- Charcot foot