Characterization of tri and tetra-nucleotide microsatellite loci for the freshwater snails Promenetus exacuous (Planorbidae) and Valvata tricarinata (Valvatidae) and their utility in population genetic studies

Yurco, Patrick; Keeney, Devon B.

doi:10.1186/s13104-018-3301-1

Table 1 Microsatellite loci developed for (a) Promenetus exacuous and (b) Valvata tricarinata

From: Characterization of tri and tetra-nucleotide microsatellite loci for the freshwater snails Promenetus exacuous (Planorbidae) and Valvata tricarinata (Valvatidae) and their utility in population genetic studies

Locus	Repeat motif	Primer sequence (5′–3′)	Label	Allele range (bp)	Round lake			Lake Saratoga
Locus	Repeat motif	Primer sequence (5′–3′)	Label	Allele range (bp)	n	A	H_O/H_E	n	A	H_O/H_E
(a) Promenetus exacuous
Pex216	(AAC)₁₁	F: CAGTCGGGCGTCATCAACTTGGAATTGGCTGCCTC	VIC	239–278	24	4	0.50/0.53	14	6	0.57/0.79
Pex216	(AAC)₁₁	R: GCAAAGGCCGGATATTTCGATC	VIC	239–278	24	4	0.50/0.53	14	6	0.57/0.79
Pex516	(AACT)₁₈	F: CAGTCGGGCGTCATCACTGTCAGAAATACGCGGCTC	6-FAM	165–261	24	10	0.75/0.86	19	10	0.84/0.89
Pex516	(AACT)₁₈	R: GGCGCGAAATGGACTAACTG	6-FAM	165–261	24	10	0.75/0.86	19	10	0.84/0.89
Pex577	(AAT)₁₀	F: GCTGTCTTTCATGGTTCCGG	6-FAM	243–276	23	1	0.00/0.00	17	4	0.41/0.47
Pex577	(AAT)₁₀	R: CAGTCGGGCGTCATCAAATGTGTCTGCAGGCGTAC	6-FAM	243–276	23	1	0.00/0.00	17	4	0.41/0.47
Pex757	(ACTC)₁₁	F: TGAGAGCCCTTAAGTCGTGG	6-FAM	169–197	24	3	0.46/0.51	19	5	0.32/0.33
Pex757	(ACTC)₁₁	R: CAGTCGGGCGTCATCAGTCAGCTACGTGATCTTGGC	6-FAM	169–197	24	3	0.46/0.51	19	5	0.32/0.33
Pex1009	(AAC)₁₂	F: TTATTGCCACTCACGTACGC	VIC	305–326	24	1	0.00/0.00	18	2	0.61/0.50
Pex1009	(AAC)₁₂	R: CAGTCGGGCGTCATCATTAACGGTTCTGGCTTCCAC	VIC	305–326	24	1	0.00/0.00	18	2	0.61/0.50
Pex1877	(AACT)₁₀	F: CAGTCGGGCGTCATCAGCTTTGGAGTATGCTTGCC	NED	244–284	24	8	0.79/0.82	19	11	0.84/0.86
Pex1877	(AACT)₁₀	R: CTAAGATTGGGAAGCCGCTG	NED	244–284	24	8	0.79/0.82	19	11	0.84/0.86
Pex2091	(AGAT)₁₆	F: CAGTCGGGCGTCATCAGAGTGTTTCGGTGCCACAG	6-FAM	272–368	24	15	0.88/0.91	18	15	0.94/0.94
Pex2091	(AGAT)₁₆	R: AAATAGTGCCGAATGTGCCG	6-FAM	272–368	24	15	0.88/0.91	18	15	0.94/0.94
Pex2117	(AACT)₉	F: CAGTCGGGCGTCATCAACCTGCAAGAAAGACCTGC	VIC	192–208	22	3	0.36*/0.63	6	3	0.33/0.70
Pex2117	(AACT)₉	R: CCTTTCCACCACATCACAGG	VIC	192–208	22	3	0.36*/0.63	6	3	0.33/0.70
Pex2181	(ACCT)₁₁	F: CAGTCGGGCGTCATCAATGTAAGTGCGTGTGTAGCC	VIC	86–162	24	2	0.17/0.22	18	9	0.89/0.84
Pex2181	(ACCT)₁₁	R: ACTTCGCGTTTGTAGGTAGG	VIC	86–162	24	2	0.17/0.22	18	9	0.89/0.84
Pex2263	(AAAG)₁₅	F: CAGTCGGGCGTCATCAGCATCCATATTTCAAAGCTGGG	PET	158–226	24	8	0.83/0.82	19	11	0.89/0.89
Pex2263	(AAAG)₁₅	R: ACTGAAGTCCCTGAAGTGGC	PET	158–226	24	8	0.83/0.82	19	11	0.89/0.89
Pex2416	(ACT)₉	F: CAGTCGGGCGTCATCATAGGGAGGCATACAAACGGAG	NED	314	24	1	0.00/0.00	19	1	0.00/0.00
Pex2416	(ACT)₉	R: TTCGCTCAATACCCAGTGATC	NED	314	24	1	0.00/0.00	19	1	0.00/0.00
Pex2471	(ACC)₈	F: AGGCAAACAGATGAGCTATGTC	PET	146–170	22	1	0.00/0.00	18	5	0.56/0.68
Pex2471	(ACC)₈	R: CAGTCGGGCGTCATCATGGTACTGGGACTTCATGGC	PET	146–170	22	1	0.00/0.00	18	5	0.56/0.68
Pex2889	(AAAG)₈	F: TACTGACTTGACGCCAATGC	PET	250–262	24	1	0.00/0.00	18	4	0.50/0.51
Pex2889	(AAAG)₈	R: CAGTCGGGCGTCATCAGTAGTCTAGGCCTTCGGTCC	PET	250–262	24	1	0.00/0.00	18	4	0.50/0.51
Pex2908	(ACCT)₁₇	F: ACCTGCATGCCTAGCTACTG	NED	128–184	24	7	0.96/0.79	19	12	0.95/0.90
Pex2908	(ACCT)₁₇	R: CAGTCGGGCGTCATCATGTCATAAATCCGGCACTGC	NED	128–184	24	7	0.96/0.79	19	12	0.95/0.90
Pex2958	(AGAT)₁₅	F: CAGTCGGGCGTCATCACTGCTATGGACGTGAGGGAG	PET	248–384	23	13	0.95/0.91	18	23	0.94/0.97
Pex2958	(AGAT)₁₅	R: TATTGATGGGCGGACGGATG	PET	248–384	23	13	0.95/0.91	18	23	0.94/0.97
Pex2972	(AACT)₁₂	F: CAGTCGGGCGTCATCAGTCATCTACGCATGGGAAGC	PET	166–226	24	5	0.79/0.80	18	11	0.76/0.85
Pex2972	(AACT)₁₂	R: GGCTTAAACTGGGACGATGC	PET	166–226	24	5	0.79/0.80	18	11	0.76/0.85
(b) Valvata tricarinata
Vtr99	(AATC)₁₂	F: CAGTCGGGCGTCATCACAGAGGTTCAAATCCCGGC	6-FAM	266–318	24	6	0.33/0.34	24	14	0.92/0.90
Vtr99	(AATC)₁₂	R: AGTTGATCATCCCGCCGTAG	6-FAM	266–318	24	6	0.33/0.34	24	14	0.92/0.90
Vtr115	(AGC)₈	F: CTTTGCCTCTTCCGGACATG	NED	127–163	24	8	0.58/0.73	24	6	0.71/0.76
Vtr115	(AGC)₈	R: CAGTCGGGCGTCATCACCTTCATTCCACCTCAGCAG	NED	127–163	24	8	0.58/0.73	24	6	0.71/0.76
Vtr565	(AAT)₂₅	F: ACGGACTACAGGTGAATACAAC	6-FAM	186–270	23	7	0.65/0.85	23	19	0.96/0.95
Vtr565	(AAT)₂₅	R: CAGTCGGGCGTCATCAGAAGTTCAATTTCGGCATGAG	6-FAM	186–270	23	7	0.65/0.85	23	19	0.96/0.95
Vtr828	(AAC)₁₁	F: CAGTCGGGCGTCATCATCTAGGGAAAGCGTGAGTGG	VIC	221–263	24	10	0.79/0.81	24	11	0.67/0.71
Vtr828	(AAC)₁₁	R: GCCCACTACAACAAGCGAAG	VIC	221–263	24	10	0.79/0.81	24	11	0.67/0.71
Vtr835	(ACT)₁₀	F: TGTCAGATCACTCTTGGGCG	PET	203–224	24	2	0.08/0.08	24	5	0.42/0.59
Vtr835	(ACT)₁₀	R: CAGTCGGGCGTCATCAACAACCTAGTGTGCCCTTC	PET	203–224	24	2	0.08/0.08	24	5	0.42/0.59
Vtr972	(AAG)₁₄	F: CTCGTTTCCTGGCTGTTGTC	NED	127–169	24	7	0.67/0.61	24	7	0.67/0.76
Vtr972	(AAG)₁₄	R: CAGTCGGGCGTCATCATAGAGTCCAAGTGTGAGGCG	NED	127–169	24	7	0.67/0.61	24	7	0.67/0.76
Vtr980	(AAG)₁₂	F: ACGCTAAGCTTTGTACAGTGC	VIC	248–296	24	8	0.63/0.75	24	6	0.38/0.40
Vtr980	(AAG)₁₂	R: CAGTCGGGCGTCATCAGAGTACCATCAAAGACGGCG	VIC	248–296	24	8	0.63/0.75	24	6	0.38/0.40
Vtr1099	(ATC)₁₀	F: CAGTCGGGCGTCATCATTCAGTGCAGACATTCGGG	VIC	258–279	24	3	0.58/0.55	24	5	0.46/0.52
Vtr1099	(ATC)₁₀	R: CTGCAGCCTCGTGAATTGAC	VIC	258–279	24	3	0.58/0.55	24	5	0.46/0.52
Vtr1279	(AAT)₁₀	F: CAGTCGGGCGTCATCAGCGAAGACAGAAATCCTCC	6-FAM	141–348	24	8	0.67/0.76	24	13	0.75/0.81
Vtr1279	(AAT)₁₀	R: ACAAATATATTTCGGTGCGCG	6-FAM	141–348	24	8	0.67/0.76	24	13	0.75/0.81
Vtr1730	(AAG)₁₉	F: CAGTCGGGCGTCATCATTGCTCCTTGGATTGGGATC	VIC	182–236	24	10	0.83/0.77	24	9	0.58*/0.76
Vtr1730	(AAG)₁₉	R: CAGCCCATTTCATCCTTGCC	VIC	182–236	24	10	0.83/0.77	24	9	0.58*/0.76
Vtr2328	(AAG)₁₀	F: CCACAGGGCCAATAAATAACTG	NED	103–118	24	2	0.29/0.25	22	3	0.45/0.59
Vtr2328	(AAG)₁₀	R: CAGTCGGGCGTCATCATAGAGTCCAAGTGTGAGGCG	NED	103–118	24	2	0.29/0.25	22	3	0.45/0.59
Vtr2349	(AAAC)₁₄	F: CAGTCGGGCGTCATCATGGGCACTGAAATCTCGTATG	VIC	324–354	24	4	0.29/0.27	13	6	0.23*/0.79
Vtr2349	(AAAC)₁₄	R: CTTACGCCACTGCCACTAAC	VIC	324–354	24	4	0.29/0.27	13	6	0.23*/0.79
Vtr2388	(AAT)₂₅	F: CAGGCCAAGATTCACACTGAC	PET	190–235	24	9	0.75/0.80	24	8	0.67/0.87
Vtr2388	(AAT)₂₅	R: CAGTCGGGCGTCATCAGTAACCCAGTCCGTGCTCG	PET	190–235	24	9	0.75/0.80	24	8	0.67/0.87
Vtr2492	(AAT)₂₀	F: CAGTCGGGCGTCATCATCTCTGCCAGCTTACCACTG	NED	227–314	24	14	0.83/0.92	24	10	0.67/0.84
Vtr2492	(AAT)₂₀	R: GGACGTTGTGCTTCTATTCTCC	NED	227–314	24	14	0.83/0.92	24	10	0.67/0.84
Vtr2508	(AAT)₉	F: CAGTCGGGCGTCATCATGTAGTGCCCATAGTCATGTAC	PET	117–132	24	2	0.50/0.42	20	2	0.20/0.39
Vtr2508	(AAT)₉	R: ACGCGTTCTCTTTAATACCTGC	PET	117–132	24	2	0.50/0.42	20	2	0.20/0.39
Vtr4154	(AAT)₉	F: CAGTCGGGCGTCATCACCTACAGATCAGAGACGTACAC	PET	209–221	24	2	0.04/0.04	24	4	0.63/0.56
Vtr4154	(AAT)₉	R: TTGCAGATCAAGGTTGTCGC	PET	209–221	24	2	0.04/0.04	24	4	0.63/0.56
Vtr4287	(AGC)₈	F: CAGTCGGGCGTCATCACCTTCATTCACCTCAGCAGC	NED	126–162	24	8	0.71/0.75	24	5	0.75/0.75
Vtr4287	(AGC)₈	R: CTTTGCCTCTTCCGGACATG	NED	126–162	24	8	0.71/0.75	24	5	0.75/0.75

Locus name is followed by repeat motif of sequenced allele, primer sequences, fluorescent dye label, total size range of alleles, and site-specific number of amplified individuals (n), number of alleles (A), and observed (H_O)/expected (H_E) heterozygosities. The 5′ primer tag is underlined and varied based on the 5′ beginning of the primer sequence. * Indicates statistically significant deviation from expected heterozygosity. GenBank Accession Numbers: P. exacuous MH000452–MH000467, V. tricarinata MH000435–MH000451

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ISSN: 1756-0500

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