Fig. 1
From: Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism
Steps employed to validate AKT1 interacters by reverse immunoprecipitation. a Few interacters were over-expressed in HEK293 cells and subjected to co-immunoprecipitation. b Western blots in panel A depict detection of BUB3 as an interacters of AKT1 in AKT1 pull down sample and parallel successful detection of AKT1 as interacters of BUB3 in BUB3 pulldown sample. Likewise, panel B depicts successful validation of GRB2 as interacters of AKT1 in AKT1 pull down sample and similarly AKT1 was found as interacters of GRB2 in GRB2 pulldown samples, respectively