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Fig. 1 | BMC Research Notes

Fig. 1

From: miRNAs in platelet-poor blood plasma and purified RNA are highly stable: a confirmatory study

Fig. 1

Stability of blood plasma miR-16-5p and miR-21-5p to freeze–thaw and room temperature incubation is associated with removal of platelets. Total RNA was isolated from PRP or PPP immediately after processing (fresh), after one through six freeze–thaw cycles of – 80 to 22 °C (FT 1–6), or after incubation of plasma at 22 °C for 24 h (RT 24). miR-16-5p and miR-21-5p levels as well as a synthetic cel-miR-39-3p spike-in (spike) were assessed by stem-loop/hydrolysis probe qPCR assays, with results presented as Cq. Data are average plus and minus standard deviation for processing replicates. Three processing replicates and three qPCR measurements are included for each condition. No RT and no template reactions were also performed, with all Cq > 37 or undetected (not shown)

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