Fig. 2
Effect of α-tocopherol on DENV2 infection of HEK293T/17 and HepG2 cells. The cytotoxicity of α-tocopherol was assessed in a uninfected HEK293T/17 (left panel) and HepG2 (right panel) and b DENV 2 infected HEK293T/17 (left panel and HepG2 (right panel) cells. Infected and uninfected cells were incubated with various concentration of α-tocopherol for 24 h and viability was assessed using MTT assay (‡absorbance value of α-tocopherol was removed from all samples to calculate MTT value). The experiments were performed independently in triplicate in parallel with control treatments and mock. A negative of cells plus H2O only was included. The standard deviation (SD) of mean are presented as error bars. c HEK293T/17 cells were infected with DENV 2 at MOI 0.5 and 2, and then treated with or without 0.5 and 1 mM of α-tocopherol or with vehicle only. d HepG2 cells were infected with DENV 2 at MOI 2 and 5 and then treated with or without 0.5 and 1 mM of α-tocopherol or with vehicle only. At 24 h post infection cells were analyzed by flow cytometry to determine the percentage infection. All experiments were undertaken independently in triplicate. Error bar showed mean ± SD (*p value ≤ 0.05)