Fig. 2

Stage customisation for environmental control, and drug addition during bioluminescence imaging. a The observation area was covered with a light-tight box, b with stage adjustments to hold two 8-well chamber slides (cyan) and eight drug reservoirs (magenta), c the automatic dispenser system inside the light-tight box. d time course of Fluo-4 fluorescence intensity in hiPSC-CM at 32, 37, and 42 °C. The measurements were triplicated at each temperature, and representative data are shown. e Temperature dependency of peak-to-peak interval of hiPSC-CM. The peak-to-peak interval decreased as temperature was raised from 32 to 42 °C. Data points show the mean ± s.e, n = 3