Fig. 1From: VEGF vascularization pathway in human intervertebral disc does not change during the disc degeneration processVEGFR1 isoforms in human degenerated and bovine healthy discs. A representative immunoblotting assay showed the expression of the full-length membrane form (~ 200 kDa), the soluble form (~ 130 kDa) and a cytoplasmic fragment (~ 60 kDa) in human degenerated (a) and healthy bovine (c) disc cells. β-Actin was used as control. Western blot quantification of membrane and soluble VEGFR1 was performed on human (b; n = 3) and bovine (d; n = 2) disc cells. Results represent an average of area (square pixels) ± SD normalized on βActin. White bars represent AF, grey bars NP. No statistical significance with non-parametric Mann–Whitney–Wilcoxon U test for independent variablesBack to article page