Table 1 Effect of in vivo passage of Chlamydia spp. stocks on Mycoplasma contamination
Species (strain) route | Isolates tested (mouse strain) | Result, in vitro pass 1 | Result, in vitro pass 2 | ||
|---|---|---|---|---|---|
MycoAlertâ„¢ | PCRa | MycoAlertâ„¢ | PCRa | ||
C. muridarum (Weiss strain) intravaginal | Culture stockb | + | + | +2 | + |
c-vc swab 1 (BALB/c) day 4 | − | − | − | nd | |
c-v swab 2 (BALB/c) day 4 | − | − | − | nd | |
c-v swab 3 (BALB/c) day 4 | − | − | − | nd | |
c-v swab 4 (C3H/HeN) day 4 | − | − | − | nd | |
c-v swab 5 (C3H/HeN) day 4 | − | − | − | nd | |
c-v swab 6 (C3H/HeN) day 4 | − | − | − | nd | |
C. trachomatis (serovar E/Bour) intravaginal | Culture stockb | + | + | + | + |
c-v swab 1 (C57BL/6) day 4 | − | − | − | − | |
c-v swab 2 (C57BL/6) day 4 | − | − | − | − | |
c-v swab 3 (C57BL/6) day 4 | − | − | − | − | |
c-v swab 4 (C57BL/6) day 4 | − | − | − | − | |
c-v swab 5 (C57BL/6) day 7 | − | − | − | − | |
c-v swab 6 (C57BL/6) day 7 | − | − | − | − | |
c-v swab 7 (C57BL/6) day 7 | − | − | − | − | |
c-v swab 8 (C57BL/6) day 7 | − | − | − | − | |
C. pneumoniae (TW-183) intranasal | Culture stockb | + | + | + | + |
Lung 1 (C57BL/6) day 4 | − | − | − | − | |
Lung 2 (C57BL/6) day 4 | − | − | − | − | |
Lung 3 (C57BL/6) day 4 | − | − | − | − | |
Lung 4 (C57BL/6) day 4 | − | − | − | − | |
Lung 5 (C57BL/6) day 7 | − | − | − | − | |
Lung 6 (C57BL/6) day 7 | − | − | − | − | |
Lung 7 (C57BL/6) day 7 | − | − | − | − | |
Lung 8 (C57BL/6) day 7 | − | − | − | − | |