Fig. 2From: Multiplex PCR for the simultaneous detection of the Enterobacterial gene wecA, the Shiga Toxin genes (stx1 and stx2) and the Intimin gene (eae)Multiplex PCR products gel electrophoresis results. a Triplex PCR performed on 23 bacterial strains of E. coli and S. dysenteriae showing the amplicon products for eae, stx and wecA genes. Lane A DNA size ladder, Lanes 1–23 bacteria as listed in Additional file 2, Lane B negative control. b Semi-nested duplex PCR using as template the stx universal PCR amplicon (526–523 bp). Lane A DNA size ladder, Lanes 1–14 stx positive strains as listed in Additional file 2 and shown in a Lanes 1–14, Lane B negative controlBack to article page