Fig. 2From: Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney diseaseDetection of AGS3 in human urinary exosomes. Spot urine samples were collected from human healthy human subjects and patients with PKD (n = 7/group). Urine samples were then centrifuged to isolate urinary exosomes. a The presence of urinary exosomes (arrows) in human urine was confirmed by electron microscopy. Scale bar = 100 nm. b Representative AGS3 expression in healthy controls and subjects with PKD or CKD was detected by Western blot analysis. c AGS3 protein expression in human urinary exosomes was measured by densitometry. Circles represent individual log2 transformed values. Line indicates group mean. *P < 0.05Back to article page