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Fig. 2 | BMC Research Notes

Fig. 2

From: Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase

Fig. 2

Maps of plasmids N-AFT/hBirA (a) and C-FTA/hBirA (b) showing the cloning of the N-terminal Avi-TEV-3xFLAG or of the C-terminal 3xFLAG-TEV-Avi tandem affinity tags under the EF1α promoter and of the hBirA-GFP under the CMV promoter of pBUDNeo. Unique restriction sites for cloning in-frame to the tags include XhoI, BglII and SfiI in N-AFT/hBirA and NotI and XhoI in C-AFT/hBirA

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BMC Research Notes

ISSN: 1756-0500

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