Fig. 2

Determination of cytotoxicity of a colored reagent by conventional MTT assays, institution of QCV assay. a Viability of cells treated with the reagent for 48 h showed haphazard pattern, while the cell pictures recorded at ×40 magnification showed dose-dependent cytotoxicity. b Dose-dependent increase in optical density was observed as a result of color of the reagent. c, d Colony number and quantitative cell number determined by dissolving crystal violet stain, and the morphology of the cells recorded at ×40 magnification correlated with each other proportionately. Statistical analysis is depicted as * < 0.05, **0.01, and *** < 0.001