Fig. 2

Effect of miR-101 on Rhes expression. Proteins or total RNAs were extracted from HEK293 cells or SH-SY5Y cells transfected with Rhes expression vector and 50 nM miRNA mimics (miR-sc or miR-101). The cells were harvested at 24 h after transfection. a Western blot analysis of Rhes expression in HEK293 cells. The immunoblots were normalized to actin expression. Data are presented as the mean ± SEM, n = 6. b Quantitative PCR analysis of miR-101 and Rhes mRNA expression in SH-SY5Y cells. Expression was quantified by real-time PCR and normalized to RNU48 or RiboL32 levels. Data are presented as the mean ± SEM, n = 3. **p < 0.01