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Table 1 Mitogen stimulation in marsupials

From: Limitations in the isolation and stimulation of splenic mononuclear cells in a dasyurid marsupial, Phascogale calura

Species

Tissue or cell type

Cell stimulant

Application

Refs.

Dendrolagus matschiei

Whole blood

PHA, ConA, PWM

[3H]-thymidine

[8]

Didelphis virginiana

Blood leukocytes

PHA, ConA, PWM

[3H]-thymidine

[13]

Macropus eugenii

PBMC, spleen MC and LNMC

PHA

[3H]-thymidine and MTT

[25]

LNMC

PHA, ConA

RNA extraction

[26, 27]

PBMC, LNMC

Zimosan, PHA, LPS, PWM

RNA extraction

[28,29,30,31]

Monodelphis domestica

PBMC

ConA, PHA, PWM

[3H]-thymidine

[32]

Whole blood, spleen, LN, thymus, peritoneal M\(\upphi\), skin

PHA, ConA, PWM, LPS

[3H]-thymidine

[33, 34]

Whole blood

ConA

[3H]-thymidine

[6]

Onychogalea fraenata

Spleen MC

PHA

RNA extraction

[31]

Perameles gunnii

PBMC

ConA, PHA, PWM, LPS

[3H]-thymidine

[35]

Phascolarctos cinereus

Whole blood, PBMC

PHA and ConA, PWM, LL, jacalin, PA and LPS

[3H]-thymidine

[9]

PBMC

Ionomycin, PMA

Flow cytometry

[36]

PBMC

PMA and ionomycin, PMA and PHA, ConA

RNA extraction

[37, 38]

PBMC

PMA and ionomycin, C. pecorum

RNA extraction

[39,40,41]

Sarcophilus harrisii

PBMC

ConA, PHA, PWM, LPS

[3H]-thymidine

[35, 42]

PBMC

Poly(I:C), LPS, flagellin, imiquimod, CpG, profiling

RNA extraction

[43]

Setonix brachyurus

PBMC

PHA

[3H]-thymidine

[44]

Blood leukocytes, spleen, thymus, LN

PHA, ConA, PWM

[3H]-thymidine

[45,46,47]

Trichosurus caninus

PBMC

Ionomycin, PMA

Flow cytometry

[36]

Trichosurus vulpecula

Spleen

PHA

[3H]-thymidine

[3, 4]

PBMC

PHA

[3H]-thymidine

[48]

PBMC, spleen, AM

M. bovis, LPS, LAM

[3H]-thymidine, RNA extraction

[49,50,51]

PBMC

Ionomycin, PMA

Flow cytometry

[36]

PBMC, spleen MC, LNMC

ConA

[3H]-thymidine, RNA extraction

[52]

  1. AM alveolar macrophages, ConA concanavalin A, CpG CpG oligodeoxynucleotide, LL lentil lectin, LN lymph nodes, LNMC lymph node mononuclear cells, LPS lipopolysaccharides, MÏ• macrophages, MC mononuclear cells, PA protein A, PBMC peripheral blood mononuclear cells, PHA phytohemaglutinin, PMA phorbol myristate acetate, PWM pokeweed mitogen