Fig. 1
From: Improving redox sensitivity of roGFP1 by incorporation of selenocysteine at position 147
Expression and spectra of roGFP (green) constructs. Constructs expressed in HEK293T. a Western blot of lysates from HEK293 cells following expression of roGFP1-N1, roGFP1-pLuc, roGFP1-Se147pLuc01, roGFP1-pSel, roGFP1-Se147pSel and a non-transfected control. β-actin (red) used as a loading control. b Excitation spectra, measured at 530 nm. c Emission spectra, excited by 405 nm