Table 1 Properties of HER2-specific VHHs isolated in this study
From: Isolation and characterization of camelid single-domain antibodies against HER2
VHH | CDR3 Length (aa)a | Human HER2 | Cynomolgus HER2 | Mouse HER2 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
kon (M−1s−1) | koff (s−1) | K D (nM) | kon (M−1s−1) | koff (s−1) | K D (nM) | kon (M−1s−1) | koff (s−1) | K D (nM) | ||
NRC-sdAb034 | 12 | 5.4 × 105 | 3.4 × 10−3 | 6.2 | 5.8 × 105 | 2.7 × 10−3 | 4.6 | 5.0 × 105 | 2.9 × 10−3 | 5.8 |
NRC-sdAb035 | 15 | 8.5 × 105 | 4.4 × 10−2 | 51 | 1.0 × 106 | 4.3 × 10−2 | 41 | n.b. | ||
NRC-sdAb036 | 19 | 3.9 × 105 | 5.6 × 10−3 | 14 | 4.8 × 105 | 5.4 × 10−3 | 11 | 4.4 × 105 | 5.8 × 10−3 | 13 |
NRC-sdAb037 | 16 | 4.3 × 105 | 1.7 × 10−2 | 39 | 5.1 × 105 | 5.6 × 10−2 | 110 | n.b. | ||
NRC-sdAb038 | 10 | 1.1 × 106 | 1.6 × 10−3 | 1.4 | 1.0 × 106 | 1.4 × 10−3 | 1.3 | 1.1 × 106 | 1.0 × 10−3 | 0.9 |
NRC-sdAb039 | 18 | 1.4 × 106 | 4.5 × 10−3 | 3.3 | 1.7 × 106 | 6.0 × 10−3 | 3.6 | n.b. | ||