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Fig. 3 | BMC Research Notes

Fig. 3

From: Validation of extracellular ligand–receptor interactions by Flow-TriCEPS

Fig. 3

Flow-TriCEPS knock down target validation of a peptide hormone (insulin) on HEK293 cells. a Flow cytometric analysis to determine binding of anti INSR Ab-PE (left panel) and anti IGF1R Ab-PE (right panel) on HEK293 cells transfected with siRNA to knock down INSR (Hs_INSR_3), IGF1R (Hs_IGF1R_8) or both receptors simultaneously for 72 h. Cells transfected with scrambled siRNA were used as control. A representative experiment out of 3 is shown. Similar results were obtained by using Hs_INSR_4 and Hs_IGF1R_6 siRNA. b Flow cytometric analysis to determine binding of INS-TriCEPS on HEK293 cells transfected with siRNA to knock down INSR (Hs_INSR_3) for 72 h (top left panel), siRNA to knock down IGF1R (Hs_IGF1R_8) for 72 h (top right panel) and siRNA to simultaneously knock down INSR and IGF1R for 72 h (low panel). Cells transfected with scrambled siRNA were used as control. A representative experiment out of 3 is shown. Similar results were obtained by using Hs_INSR_4 and Hs_IGF1R_6 siRNA

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