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Fig. 2 | BMC Research Notes

Fig. 2

From: Antiviral response elicited against avian influenza virus infection following activation of toll-like receptor (TLR)7 signaling pathway is attributable to interleukin (IL)-1β production

Fig. 2

TLR7 ligand, ssRNA elicits antiviral response against H4N6 LPAIV in macrophages but not attributable to ssRNA-mediated NO production. a Avian macrophages, MQ-NCSU cells, were cultured in 12-well plates for 24 h and stimulated with either ssRNA (10 µg/ml), ssRNA (10 µg/ml) plus 1400 W (100 µM), 1400 W (100 µM) or growth medium (control) in triplicate. The cell culture supernatants were collected 24 h post-treatment, a portion was transferred onto MDCK cell mono layers grown in 12-well plates and infected with H4N6 LPAIV (50 PFU/well). b The NO assay was performed in remaining cell culture supernatants using Griess assay to quantify NO production. The experiment was repeated two more times with the same number of replicates with similar results and the data were pooled. The one-way ANOVA test followed by Bonferroni’s posttest for selected comparison was performed to identify group differences and the differences were considered significant at P < 0.05. The bars represent mean ± SEM

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