Fig. 2

TLR2 plays a key role in the antitumor efficacy of MIP. Wild-type and TLR knockout mice were implanted with B16F10 tumor cells in their right flank, treated with MIP and monitored for tumor growth. Lower tumor volume were observed in MIP-treated wild-type mice, compared with PBS-treated control animals (a, b). MIP-treated TLR2^−/− mice developed large size tumors, as developed by PBS-treated wild-type animals (c). MIP-treated TLR4^−/− mice developed tumors comparable with those in MIP-treated wild-type animals (d). Each curve represents tumor volume in an individual animal. Mean ± SEM of tumor volume in different animal groups on day 21 are shown in the form of bar graph (e). Representative data of the one of two independent experiments are shown