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Fig. 1 | BMC Research Notes

Fig. 1

From: Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Fig. 1

Activities of glucose metabolic enzymes in Caco2, HaCaT, C2C12, HEK-293, HepG2, and INS-1E cells. a Aldolase (Aldo), b Hexokinase (low Km) (HK), c Glucose 6 phosphate dehydrogenase (G6PD), d Phosphoglucose isomerase (PGI), e Phosphofructokinase (PFK) and f Phosphoglucomutase (PGM). N = 4–6 extracts tested per cell line. Data are shown as mean ± SEM. Testing of parameters on aldolase activity assays in INS-1E samples: g Dilution series of cell extracts (N = 3). Diluted samples were normalized to the level of the undiluted sample (set to 1). h Temperature sensitivity was tested by assay performance at 25 °C and 30 °C. N = 4 cell extracts tested per temperature. i Sensitivity to freeze–thaw cycles was assessed using cell extracts that underwent 1 (I), 2 (II) or 3 (III) freeze-thaw cycles. N = 4 cell extracts tested per condition. Data are shown as mean ± SEM. Additional data relating to this figure can be found in Additional file 2

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