Fig. 2From: Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility studyEnzyme activities and mRNA levels of aldolase of INS-1E cells treated with palmitate for 24 h. INS-1E cells were treated with palmitate or BSA for 24 h. a Cellular lysates were prepared and used to determine enzymatic activity of aldolase (Aldo). Raw activities were corrected for protein concentration. b Messenger RNA levels of AldoA was determined using RT-Q-PCR. Data are presented as mean fold change with controls set as 1, and are shown as mean ± SEM, N = 5–7 samples. *p < 0.05Back to article page