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Table 1 Enzyme assay performances

From: Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study

Enzyme assay performance
Enzyme Mean V (nmol/s/mg prot) Standard deviation N Intra assay CV (%)
Aldolase 1.07 0.08 4 7.7
G6PD 0.16 0.01 4 7.2
PGI 12.0 1.9 3 15.7
PGM 0.81 0.07 3 9.2
Performance of entire experimental procedure
Enzyme Mean V (nmol/s/mg prot) Standard deviation N Sample CV (%)
Aldolase 1.07 0.16 12 16.1
G6PD 0.16 0.06 11 14.6
PGI 12.0 2.0 9 16.4
PGM 0.84 0.12 9 14.8
  1. Aldo Average enzymatic activities of aldolase, G6PD lucose 6 phosphate dehydrogenase, PGI phosphoglucose isomerase and PGM phosphoglucomutase determined in INS-1E cell lysate, and average enzymatic activities of Aldo, G6PD, PGI and PGM determined in biological replicates of INS-1E cell extracts derived from 3 different cell passages